Total T4 ELISA Kit
The TRUSTwell T4 ELISA Kit is a solid-phase enzyme-linked immunosorbent assay for the quantitative measurement of total Thyroxine (T4) in human serum.
- Catalog Number: AE1020
- Brand: TRUSTwell
- Pack Size: 96 well
- Shelf Life: 18 months
- Specimen Type: Serum
- Storage Temperature: 2-8 °C
₹5,000.00 Original price was: ₹5,000.00.₹2,605.00Current price is: ₹2,605.00.
The TRUSTwell T4 ELISA Kit is a solid-phase enzyme-linked immunosorbent assay for the quantitative measurement of total Thyroxine (T4) in human serum.
Features of Total T4 ELISA Kit:
- Microwells coated with anti-T4 antibody 1 plate, 96 wells
- T4 Standards: S1 (0 µg/dl) 1 mL
- T4 Standards: S2 (2.0 µg/dl) 1 mL
- T4 Standards: S3 (5.0 µg/dl) 1 mL
- T4 Standards: S4 (10.0 µg/dl) 1 mL
- T4 Standards: S5 (15.0 µg/dl) 1 mL
- T4 Standards: S6 (25.0 µg/dl) 1 mL
- HRP-T4 Conjugate Concentrate (20X) 1.2 mL
- Enzyme Conjugate Diluent 2 Vials, 7.5 mL
- Wash Buffer Concentrate (40X) 25 mL
- Substrate 12.0 mL
- Stop solution 7.5 mL
- The product inserts 1 set
Intended use of Total T4 ELISA Kit:
The Total Thyroxine (T4) ELISA is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of T4 concentration in human serum. It is intended to be used by professionals as an aid in the diagnosis of thyroid dysfunction.
Introduction of TRUSTwell Total T4 ELISA Kit:
Thyroid disease is very common on a global level and studies have shown that the prevalence of undiagnosed thyroid disorder is high. Thus, quantification of markers that reflect thyroid function is very important. Thyroid function and metabolism are controlled by the human hypothalamic-pituitary-thyroid (HPT) axis, which is made up of the hypothalamus, the pituitary gland, and the thyroid. Thyroid-stimulating hormone (TSH) controls the production of the thyroid hormones thyroxine (T4) and 3, 5, 3’ triiodothyronine (T3), which play an important role in the regulation of metabolism.
The majority of T3 and T4 that circulate in the blood are protein-bound and attach to plasma proteins such as Thyroxine-Binding Globulin (TBG). The small percentage that is not protein-bound is considered “free” (fT4/fT3), and can readily enter target tissue. Importantly, the binding protein concentration of each individual can be affected by factors such as pregnancy, hormonal therapy, and certain medications, which will, therefore, affect T4/T3 test results. Thus, testing of thyroid function commonly encompasses TSH and a combination of the thyroid hormones (T3/T4, fT3/fT4) assays.
T4 tests can be used to aid in the detection and diagnosis of thyroid disease including hyperthyroidism, hypothyroidism, and Graves’ disease, and to monitor pregnancy and treatment for thyroid disorder, pituitary disease, and thyroid cancer. T4 measurements are generally employed in conjunction with results from other
members of the thyroid function panel as well as a measurement or estimate of binding protein levels such as TBG to give the most accurate measurement of thyroid status possible.
TRUSTwell Total T4 ELISA Kit:
The Total T4 ELISA Kit is a solid-phase competitive Enzyme-Linked Immunosorbent Assay (ELISA) designed to quantitatively measure thyroxine (T4) present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. Here are some key details about this kit:
- Analytical Sensitivity: The kit can detect T4 at a sensitivity of 0.29 ng/mL.
- Assay Range: It covers a range from 0.625 ng/mL to 20 ng/mL.
- Sample Types and Volumes:
- Serum: 1 µL
- Plasma: 1 µL
- Urine: 2.5 µL
- Fecal Extract: 10 µL
- Supernatant: 10 µL
- Hands-on Time: Approximately 1 hour and 20 minutes.
- Time-to-Result: The total time required for results is around 1 hour and 30 minutes.
- Homogeneous (No Wash): No washing steps are involved.
- Interassay CV: Less than 7%.
- Intraassay CV: Less than 3%.
- Instrument: Colorimetric Microplate Reader.
- Product Size: Each kit contains materials for 96 tests.
- Contents: The kit includes a pre-coated 96-well plate, standard, assay diluent concentrate, HRP-conjugated detection antibody, wash buffer, chromogen, stop solution, and adhesive plate covers.
- Storage: Store the kit at -20°C.
Target Information:
- Thyroxine (T4) is the main hormone produced by the thyroid gland.
- T4, along with triiodothyronine (T3), regulates metabolism and plays a crucial role in growth and development.
- Iodine is necessary for the production of T3 and T4.
Weight | 0.450 kg |
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Dimensions | 18 × 12 × 12 cm |
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