Free T3 ELISA Kit
The TRUSTwell Free T3 ELISA Kit is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of free triiodothyronine (fT3) levels in human serum.
- Catalog Number: AE1016
- Brand: TRUSTwell
- Pack Size: 96 well
- Shelf Life: 12 months
- Specimen Type: Serum
- Storage Temperature: 2-8 °C
₹6,000.00 Original price was: ₹6,000.00.₹2,977.00Current price is: ₹2,977.00.
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The TRUSTwell Free T3 ELISA Kit is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of free triiodothyronine (fT3) levels in human serum.
Features of Free T3 ELISA Kit:
- Sensitivity: 274 ± 0.077 pg/mL at 95% confidence level
- Specificity: <0.001 cross-reactivity with l-thyroxine at 10 µg/mL, iodotyrosine at 10 µg/mL, diiodotyrosine at 10 µg/mL, and d-thyroxine at 10 µg/mL, respectively
- Accuracy: correlation coefficient 0.958 ± 0.087 at 95% confidence level
- Intra Assay Precision: CV < 5.1%
- Inter Assay Precision: CV < 7.5%
- Shelf life: 18 months
- Open kit stability: 8 weeks at 2-8 °C, or until the expiration date
- Anti-fT3 Ab Coated Microwells
- fT3 Calibrators C1-C6
- HRP-T3 Conjugate
- Wash Buffer (30X Concentrate)
- TMB Substrate
- Stop Solution
- ELISA Working Sheet
Intended use of Free T3 ELISA Kit:
The Free Triiodothyronine (fT3) ELISA is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of fT3 concentration in human serum. It is intended to be used by professionals as an aid in the diagnosis of thyroid dysfunction.
Introduction of TRUSTwell Free T3 ELISA Kit:
Thyroid disease is very common on a global level and studies have shown that the prevalence of undiagnosed thyroid disorder is high. Thus, the quantification of markers that reflect thyroid function is very important. Thyroid function and metabolism are controlled by the human hypothalamic-pituitary-thyroid (HPT) axis, which is made up of the hypothalamus, the pituitary gland, and the thyroid. Thyroid-stimulating hormone (TSH) controls the production of the thyroid hormone thyroxine (T4) and 3,5,3’ triiodothyronine (T3), which play an important role in the regulation of metabolism.
The majority of T3 and T4 that circulate in the blood are protein-bound and attach to plasma proteins such as Thyroxine-Binding Globulin (TBG). The small percentage that is not protein-bound is considered “free” (fT4/fT3), and can readily enter target tissue. Importantly, the binding protein concentration of each individual can be affected by factors such as pregnancy, hormonal therapy, and certain medications, which will, therefore, affect T4/T3 concentrations. Due to the general stability of the “free” thyroid hormone concentrations, fT4/fT3 test results may be considered more useful in many cases.
Testing of thyroid function commonly incorporates TSH testing along with a combination of T3, T4, fT3, and fT4 assays3,7,8. Generally, and depending on the status of the individual’s TSH concentration, elevated fT3 results may indicate hyperthyroidism, and low fT3 results may indicate hypothyroidism. Free T4 tests are also commonly used to monitor pregnancy and certain treatments for thyroid disorder, pituitary disease, and thyroid cancer8,9. Combining fT3 with other thyroid hormone results and any other necessary test results will help provide enough information for the healthcare provider to design a proper plan of care.
TRUSTwell Free T3 ELISA Kit:
The Free T3 ELISA Kit is intended for the quantitative determination of Free T3 (Triiodothyronine) in serum by enzyme-linked immunoassay (ELISA). It is designed for research use only and not for diagnostic procedures. Here are some key details about this kit:
- Sample Type: It works with human serum.
- Detection Method: Utilizes a colorimetric technique based on ELISA.
- Assay Type: The assay follows a solid-phase format for qualitative detection.
- Clinical Significance:
- Triiodothyronine (T3) is one of the thyroid gland hormones.
- Among the thyroid hormones produced in the thyroid gland, T3 is regarded as the most biologically active molecule.
- Hyperthyroidism is generally associated with an increase in FT3 concentration.
- The measurement of free T3 (FT3) is necessary for the follow-up of patients undergoing therapy with triiodothyronine.
- The present 1-step free T3 radioimmunoassay enables a reliable determination of FT3 in the concentration range from 0 to 40 pmol/l (0 – 26 pg/ml).
Weight | 0.450 kg |
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Dimensions | 18 × 12 × 12 cm |
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