Vitamin D (Total) ELISA Kit
TRUSTwell Vitamin D (Total) ELISA is a solid-phase enzyme-linked immunosorbent assay for the quantitative determination of Vitamin D (Vit D) levels in human serum.
- Catalog Number: AE8010
- Brand: TRUSTwell
- Pack Size: 96 well
- Shelf Life: 18 months
- Specimen Type: Serum
- Storage Temperature: 2-8 °C
₹21,000.00 Original price was: ₹21,000.00.₹12,204.00Current price is: ₹12,204.00.
42% Off
TRUSTwell Vitamin D (Total) ELISA is a solid-phase enzyme-linked immunosorbent assay for the quantitative determination of Vitamin D (Vit D) levels in human serum.
Features of Vitamin D (Total) ELISA Kit:
- Accuracy: R2 = 0.918 with CLIA as reference.
- Analytical Sensitivity: 1.2 ng/mL.
- Specificity: No cross-reactivity to non-hydroxylated vitamin D2 and Vitamin D3.
- Intra & Inter-assay precision: CV <=10%.
- Higher linearity: 0-150 ng/mL.
- 1 step less procedure, faster result.
- High & Low level assay controls included.
- Accuracy: R2 = 0.918 with CLIA as reference.
- Analytical Sensitivity: 1.2 ng/mL.
- Specificity: No cross-reactivity to non-hydroxylated vitamin D2 and Vitamin D3.
- Intra & Inter-assay precision: CV <=10%.
- Higher linearity: 0-150 ng/mL.
- 1 step less procedure, faster result.
- High & Low level assay controls included.
Intended use of Vitamin D (Total) ELISA Kit:
The TRUSTwell Vitamin-D (Total) ELISA is a solid-phase enzyme-linked immunosorbent assay for the quantitative determination of Vitamin D (Vit D) levels in human serum. It is intended to be used by trained professionals only.
Introduction of TRUSTwell Vitamin-D (Total) ELISA Kit:
Vitamin D is a fat-soluble steroid hormone that is ingested through diet and synthesized in the skin, liver, and kidneys after exposure to sunlight1. Two main forms of Vitamin D are found in the diet, Vitamin D3 (cholecalciferol) and Vitamin D2 (ergocalciferol). Vitamin D3, however, is more effective at regulating Vitamin D levels than Vitamin D2.
Vitamin D plays an important role regulation of calcium and phosphorus in the human body. It is important for the maintenance of healthy bones and teeth and for support of immune, nervous, endocrine, and cardiovascular system health. Vitamin D deficiency is a global health issue and is one of the most common nutrient deficiencies. It is more prevalent in females than males and varies according to ethnicity and eating habits. Vitamin D deficiency has been associated with rickets, osteomalacia, and osteoporosis, as well as fetal development, immune system function, neurological disorders such as multiple sclerosis, cancer, and many other pathologies. Therefore, accurate measurement of Vitamin D levels in patients is very important for the diagnosis of Vitamin D deficiency and the monitoring of deficient patients to achieve optimal levels.
For Vitamin D to become “active”, it goes through two conversion steps. In the liver, it is first converted to 25-hydroxy-vitamin D (25(OH)D), which is the “storage” and major circulating form of Vitamin D in the body. It is next converted to the “active” form of Vitamin D, 1, 25-hydroxy calcitriol (1,25(OH)2D), in the kidneys. 1,25(OH)2D has a short half-life, however, making detection less accurate. Because 25(OH)D is the major circulating form and stays constant in the body for longer, it is the most accurate and therefore most common biomarker of Vitamin D status. The sum of 25(OH)D in serum is referred to as a total of 25(OH) Vitamin D.
TRUSTwell Vitamin D (Total) ELISA Kit:
The Vitamin D ELISA kit is a complete assay designed for the quantitative determination of 25(OH) Vitamin D3 and 25(OH) Vitamin D2 in human plasma and serum samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique and provides a reliable method for assessing total Vitamin D concentration. Here are some key details about this kit:
- Sensitivity: The kit can detect 25(OH) Vitamin D at a sensitivity of 1.98 ng/ml.
- Assay Range: It covers a range from 0.5 ng/ml to 1010 ng/ml.
- Sample Types: You can use plasma and serum samples.
- Assay Type: Competitive ELISA.
- Detection Method: Colorimetric.
- Assay Duration: Approximately 1 hour and 30 minutes.
- Species Reactivity: Reacts with humans.
- Importance of 25(OH) Vitamin D Measurement:
- Vitamin D levels are associated with various disease states and indicators of well-being.
- These include bone diseases (such as osteoporosis and arthritis) and other conditions like hypertension, diabetes, cancer, and heart disease.
- The detection of the 25(OH) Vitamin D metabolite serves as an indicator for total Vitamin D concentration.
- Transformation to Active Form:
- UV rays from the sun act on 7-dehydrocholesterol to form parent Vitamin D3.
- Alternatively, Vitamin D can be ingested as parent Vitamin D2 from food sources.
- These parent compounds undergo hydroxylation to 25(OH) Vitamin D, which is then transported to the kidney for further hydroxylation to the biologically active form, 1,25(OH)2 Vitamin D.
Weight | 0.450 kg |
---|---|
Dimensions | 18 × 12 × 12 cm |
Promotional Information
Related products
HIV 1+2 Ab ELISA Kit
TSH ELISA Kit
Dengue IgM ELISA Kit
Dengue Ag (NS1) ELISA Kit
Syphilis Ab ELISA Kit
TRUSTwell Ferritin ELISA Kit
HCV Total Ab ELISA Kit
Dengue IgG ELISA Kit
ALTA ELISA Plate Reader
ALTA ELISA Washer
CHIK IgM ELISA Kit
FAST SHIPPING
We offered fast delivery depends on stock availability and location.
ORDER RETURN
Return within 3 days
ORDER RETURN Return within 3 days with proper storage condition If for any reason, you want to return the product, you are free to return it at the time of delivery. However, in such return cases the cancellation rules will be applicable. The return request would need to be sent through the e-mail from your registered e-mail ID, at ecommerce@athenesedx.com, or by contacting us on our Sales Support Phone No +91 7397238924, mentioning order details being mandatory.
Reviews
There are no reviews yet