The TRUSTwell Total T3 ELISA Kit is to be used for the quantitative determination of total Triiodothyronine (T3) levels in human serum.
Features of Total T3 ELISA Kit:
- Detection Method: Utilizes a colorimetric technique based on the ELISA (Enzyme-Linked Immunosorbent Assay).
- Assay Type: The assay follows a solid-phase format for qualitative detection.
- Sample Type: Works with human serum or plasma.
- Sensitivity: The detection limit is approximately 0.2 ng/mL of T3.
- Storage: Store at 4°C.
Each product kit contains:
- Microwells coated with T3 analog 1 plate, 96 wells
- T3 Standards: S1 (0 ng/ml) 1 mL
- T3 Standards: S2 (0.5 ng/ml) 1 mL
- T3 Standards: S3 (1.0 ng/ml) 1 mL
- T3 Standards: S4 (2.5 ng/ml) 1 mL
- T3 Standards: S5 (5.0 ng/ml) 1 mL
- T3 Standards: S6 (10 ng/ml) 1 mL
- HRP-anti-T3 Antibody Conjugates (20X Concentrate) 1.2 mL
- Enzyme Conjugate Diluent 2 vials, 7.5 mL/Ea
- Wash Buffer Concentrate (40X) 25 mL
- Substrate 12.0 mL
- Stop solution 7.5 mL
- The product inserts 1 set
Intended use of Total T3 ELISA Kit:
The Total Triiodothyronine (T3) ELISA is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of T3 concentration in human serum. It is intended to be used by professionals as an aid in the diagnosis of thyroid dysfunction.
Introduction of TRUSTwell Total T3 ELISA Kit:
Thyroid disease is very common on a global level and studies have shown that the prevalence of undiagnosed thyroid disorder is high. Thus, quantification of markers that reflect thyroid function is very important. Thyroid function and metabolism are controlled by the human hypothalamic-pituitary-thyroid (HPT) axis, which is made up of the hypothalamus, the pituitary gland, and the thyroid. Thyroid-stimulating hormone (TSH) controls the production of the thyroid hormones thyroxine (T4) and 3, 5, 3’ triiodothyronine (T3), which play an important role in the regulation of metabolism.
The majority of T3 and T4 that circulate in the blood are protein-bound and attach to plasma proteins such as Thyroxine-Binding Globulin (TBG). The small percentage that is not protein-bound is considered “free” (fT4/fT3), and can readily enter target tissue. Importantly, the binding protein concentration of each individual can be affected by factors such as pregnancy, hormonal therapy, and certain medications, which will, therefore, affect T4/T3 test results. Thus, testing of thyroid function commonly encompasses TSH and a combination of the thyroid hormones (T3/T4, fT3/fT4) assays.
Total T3 tests can be used to aid in the detection and diagnosis of thyroid disease including hyperthyroidism, hypothyroidism, and Graves’ disease, and to monitor pregnancy and treatment for thyroid disorder, pituitary disease, and thyroid cancer. T3 measurements are generally employed in conjunction with results from other
members of the thyroid function panel as well as a measurement or estimate of binding protein levels such as TBG to give the most accurate measurement of thyroid status possible.
TRUSTwell Total T3 ELISA Kit:
The Total T3 ELISA Kit is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed for the qualitative detection of total triiodothyronine (T3) in human serum or plasma.
- Purpose:
- Detects total T3, which includes both free and protein-bound forms.
- Useful for assessing thyroid function and diagnosing thyroid disorders.
- Sample Type: It works with human serum or plasma.
- Detection Method: Utilizes a colorimetric technique based on the ELISA.
- Assay Type: The assay follows a solid-phase format for qualitative detection.
- Clinical Significance:
- T3 is a thyroid hormone that plays a crucial role in metabolism, growth, and development.
- Abnormal T3 levels can indicate hyperthyroidism, hypothyroidism, or other thyroid-related conditions.
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