TRUEchemie offers System pack reagents for Autochem 200 with high quality and exceptional performance. The TRUEchemie RF / RA (Rheumatoid Factor / Rheumatoid Arthritis) Test Kit (System Pack) is for the qualitative and semi-quantitative determination of RF/RA in serum.
Features of RA/RF Test Kit (SP):
- Used for qualitative and semi-quantitative determination of RF in Serum
- Rapid screening within 2 minutes
- Highly stabilized latex reagent
- Distinct agglutination with a sensitivity of 12 IU/mL
- Packed with positive and negative controls.
- Necessary accessories provided.
Intended use of RA/RF Test Kit (SP):
The TRUEchemie Rheumatoid Factor (RF) Test Kit (SP) is used for the qualitative and semi-quantitative determination of the Rheumatoid Factor (RF) in serum.
Introduction of RA/RF Test Kit (SP):
Measurement of rheumatoid factor is used for differentiating rheumatoid arthritis from other Chronic inflammatory arthritis and is important in the progress and therapeutic management of the disease. Rheumatoid factor has been associated with some bacterial and viral infections (eg. Hepatitis, infectious Mononucleosis) Some Chronic infections (e.g.Tuberculosis, Parasitic diseases, Subacute Bacterial Endocarditis), and Cancer.
TRUEchemie RA/RF Test Kit (SP):
The RF-Turbilatex is a quantitative turbidimetric test used for measuring Rheumatoid Factors (RF) in human serum or plasma.
- Principle of the Method:
- The test uses latex particles coated with human gammaglobulin.
- When mixed with samples containing RF, these latex particles agglutinate.
- The agglutination causes an absorbance change, which depends on the RF content in the sample.
- By comparing this change to a calibrator with a known RF concentration, the test quantifies the RF level.
- Clinical Significance:
- Rheumatoid factors are antibodies directed to determinants in the Fc portion of the immunoglobulin G molecule.
- While they are found in various rheumatoid disorders (e.g., systemic lupus erythematosus, Sjögren’s syndrome), their central role lies in aiding the diagnosis of rheumatoid arthritis (RA).
- A study by the “American College of Rheumatology” found that 80.4% of RA patients were RF positive.
- Reagents:
- Diluent (R1): Used to prepare the sample.
- Latex (R2): Coated with human gammaglobulin.
- RF-CAL Calibrator: Contains human serum with a known RF concentration.
- Calibration:
- Use the RF Calibrator Reference 1107007.
- The assay sensitivity and target value are standardized against the International Reference NIBSC 64/2 (Rheumatoid Arthritis Serum) WHO.
- Recalibrate when control results are out of specified tolerances or when using a different reagent lot.
- Sample Preparation:
- Use a fresh serum.
- Centrifuge samples with fibrin before testing.
- Avoid highly hemolyzed or lipemic samples.
- Reference Values:
- Normal values are up to 20 IU/mL, but each laboratory should establish its own reference range.
- Quality Control:
- Control Sera is recommended before and after testing samples to monitor performance.
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