Diluent Reagent

TRUEchemie Diluent Reagent:

The Diluent Reagent plays a crucial role in the operation of a hematology analyzer, particularly for blood cell analysis. Let’s delve into the details:

1. Purpose of Diluent Reagent:
   • The Diluent Reagent is used to dilute blood samples before analysis in the hematology analyzer.
   • It ensures that blood cells remain in their original shape during testing.
   • Dilution helps maintain the essential properties of blood cells, such as osmolarity, pH, conductivity, and cushioning effect.
2. Composition of Diluent Reagent:
   • The typical composition of the diluent for a hematology analyzer includes:
     • Sodium chloride: Maintains osmolarity.
     • Sodium sulfate: Helps stabilize the solution.
     • Phosphate buffer agent: Maintains pH.
     • Ethylene diamine tetraacetic acid (EDTA): Prevents clotting.
     • 1-pyridone-2-sulphur: Enhances stability.
     • Formaldehyde: Preserves cell integrity.
   • The pH value of the solution is usually maintained between 6.5 and 7.4, preferably around 7.2.
3. Function of Diluent Reagent:
   • The diluent creates a stable environment for blood cell count within the hematology analyzer.
   • It ensures accurate results by providing consistent conditions for cell analysis.
   • Dilution prevents cell aggregation and allows precise measurement of parameters like hemoglobin, white blood cell count, and platelets.
4. Usage and Recommendations:
   • Store the diluent in a dark place to avoid exposure to sunlight.
   • Avoid contact with skin and eyes. In case of contact, rinse immediately with water and seek medical advice.
   • The diluent is ready to use and should be tightly closed after each use.
   • Do not freeze the reagent.
   • Always use the diluent under professional guidance.