TRUEchemie Bilirubin Total Test Kit (System Pack) for the quantitative determination of total bilirubin in human serum.
Features of Bilirubin Total Test Kit (SP):
- Reliable
- Accurate
- Cost-effective
- Preferred by lab technicians worldwide.
- Method: SYSTEM PACK
Intended use of Bilirubin Total Test Kit (SP):
TRUEchemie Bilirubin Total Test Kit (SP) for the quantitative determination of total bilirubin in human serum.
Introduction of Bilirubin Total Test Kit (SP):
Bilirubin is a metabolite of the heme portion of heme proteins, mainly hemoglobin. Normally it is excreted into the intestine and bile from the liver. The site of the catabolism of hemoglobin is the reticuloendothelial system (RES). Bilirubin is then released into the bloodstream where it binds tightly to albumin and is transported to the liver. Upon uptake by the liver, bilirubin is conjugated with glucuronic acid to form bilirubin mono and diglucuronide which are water-soluble metabolites.
The metabolites will react with aqueous diazo reagent and are commonly referred to as “direct bilirubin”. Elevation of total serum bilirubin may occur due to,
- excessive hemolysis or destruction of the red blood cells e.g. hemolytic disease of the newborn,
- liver diseases e.g. hepatitis and cirrhosis
- obstruction of the biliary tract e.g., gallstones.
There is information in the literature indicating elevated levels of direct bilirubin in patients with liver or biliary tract disease, even though, total bilirubin levels are normal. Therefore, the greatest diagnostic value of direct bilirubin assays stems from their ability to indicate occult liver disease.
Most chemical methods for the determination of total bilirubin are based on the reaction between diazotized sulfanilic acid and bilirubin to produce azobilirubin, which absorbs maximally at 546 nm.
TRUEchemie Bilirubin Total Test Kit (SP):
The Bilirubin Assay Kit (Total and Direct, Colorimetric) is a laboratory test designed to quantitatively measure both total bilirubin (unconjugated + conjugated) and direct bilirubin (conjugated) in biological samples such as serum. Here are some key details about this kit:
- Principle: The kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo-salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin is determined in the absence of a catalyst (at 550 nm).
- Importance of Bilirubin Measurement:
- Bilirubin is a degradation product of heme catabolism.
- It exists in two forms: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin.
- Bilirubin concentration in the body depends on various factors.
- Low serum bilirubin is correlated with conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases.
- High bilirubin indicates hemolysis, jaundice, Gilbert’s syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts.
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